Plasminogen, Antithrombin Xa and Protein C from Helena Biosciences
The Antithrombin Xa kit is a chromogenic assay intended for the quantitative determination of Antithrombin III (AT-lll) activity in human citrated plasma. This two-stage method utilises Factor Xa, added to a patient plasma dilution containing AT-lll in the presence of excess heparin and calcium. After an initial incubation period (stage 1), residual Factor Xa is determined with a Factor Xa-specific chromogenic substrate (stage 2). The residual Factor Xa activity is inversely proportional to the AT-lll concentration.
The Protein C kit is intended for the quantitative determination of Protein C in citrated human plasma. Protein C in the patient plasma is activated by a specific fraction from the Agkistrodon Contortrix snake venom (Protac®). The amount of activated protein C (APC) is determined by monitoring the rate of hydrolysis of a Protein C specific chromogenic substrate. The release of pNA is measured and is proportional to the Protein C level in the patient’s plasma.